Journal: Autophagy

Article Title: CHCHD2 and CHCHD10 promoted autophagic clearance of protein aggregates via GABARAPs

doi: 10.1080/15548627.2026.2678427

Figure Lengend Snippet: Plasmids.

Article Snippet: MYC-LAMP2A , Origene TM Technologies , RC221216.

Techniques: Plasmid Preparation

Journal: Journal of Nanobiotechnology

Article Title: Delivery of LOXL1-AS1 -siRNAs using targeting peptide-engineered extracellular vesicles with focused ultrasound to suppress medulloblastoma metastasis

doi: 10.1186/s12951-025-03554-0

Figure Lengend Snippet: Expression vector design of a medulloblastoma-specific peptide for stable expression in human mesenchymal stem cells (MSCs) and derived extracellular vesicles (EVs). (A) Schematic illustration of the construct and experimental design. (B) Flow cytometry of different cell lines after 1 h of incubation with synthetic peptides. Population comparison values (% positive) of the E1-3 peptide versus the NC peptide are indicated on each graph. (C) Brightfield microscopic images of MSCs after 48 h of culture in serum-free medium for EV collection. Scale bars, 100 μm. (D) Cell viability after medium collection, presented as the percentage of living cells relative to the total number of cells counted. (E) RT-qPCR analysis of a housekeeping gene (HSPCB) and two fusion transcripts ( Lamp2-V5 and Lamp2-E1-3) in MSCs. A forward primer located at the V5-tag or E1-3 peptide sequence and a reverse primer located at the Lamp2 sequence were used to detect the fusion transcripts. Expression levels are relative to the Lamp2-E1-3 group. (F) Western blot analysis of Lamp2 and V5-tag expressions in MSCs and their derived EVs, with quantification values normalized to β-actin and relative to the parental group provided below each band. Quantitative data are presented as the mean ± SD of replicates from representative of three independent experiments. nd, non-detected

Article Snippet: Complementary (c)DNA of human Lamp2 variant B was purchased from OriGene (cat. no. RC200456, Rockville, MD, USA).

Techniques: Expressing, Plasmid Preparation, Derivative Assay, Construct, Flow Cytometry, Incubation, Comparison, Quantitative RT-PCR, Sequencing, Western Blot

Journal: Journal of Nanobiotechnology

Article Title: Delivery of LOXL1-AS1 -siRNAs using targeting peptide-engineered extracellular vesicles with focused ultrasound to suppress medulloblastoma metastasis

doi: 10.1186/s12951-025-03554-0

Figure Lengend Snippet: Characterization of medulloblastoma-targeted extracellular vesicles (EVs). Three stable lines (Parental, Lamp2-V5, and Lamp2-E1-3) of mesenchymal stem cells (MSCs) and their derived EVs were analyzed. (A) EV yield of MSCs as the EV-producing cells, calculated by dividing the total number of isolated EVs by the total number of MSCs. (B) EV purity ratios, calculated by dividing the total protein extracted from EVs (µg) by the total EV number. (C) EV diameters in nanometers (nm), presented as the mode value of particle sizes measured by nanoparticle tracking analysis (NTA). (D) Size distribution by NTA of EV samples from each MSC line. (E) Transmission electron microscopy (TEM) of EV samples from each MSC line. EV structures presented in the image are indicated with black arrowheads. Scale bars, 100 nm. (F) Western blot analysis of MSCs and their EVs for multiple EV-associated markers, with quantification values relative to the parental group provided below each band. Quantitative data are presented as the mean ± SD of samples from three independent experiments. ns, non-significant

Article Snippet: Complementary (c)DNA of human Lamp2 variant B was purchased from OriGene (cat. no. RC200456, Rockville, MD, USA).

Techniques: Derivative Assay, Isolation, Transmission Assay, Electron Microscopy, Western Blot

Journal: Journal of Nanobiotechnology

Article Title: Delivery of LOXL1-AS1 -siRNAs using targeting peptide-engineered extracellular vesicles with focused ultrasound to suppress medulloblastoma metastasis

doi: 10.1186/s12951-025-03554-0

Figure Lengend Snippet: In vivo tracking of intravenously administered medulloblastoma-targeted extracellular vesicles (EVs) in combination with microbubble-enhanced focused ultrasound (FUS). ( A - B ) In vivo tracking of EVs with FUS. See also Supplementary Figure A. (A) Fluorescence imaging performed at 1 h after EV injection (left) and quantification of EV signals in the head and body regions, normalized to the background signal of PBS and relative to EV without FUS (right). (B) Fluorescence imaging of internal organs obtained immediately after humane euthanasia (upper) and quantification of EV signals in each organ, normalized to the background signal of PBS and relative to EV without FUS (lower). ( C - F ) In vivo tracking of EVs expressing the E1-3 peptide. See also Supplementary Figure B. ( C - D ) Serial fluorescence imaging performed every 10 min until 2 h after EV injection, including (C) representative images at indicated time points and (D) quantification of EV signals. Statistical analyses between the Lamp2-NC EV + FUS and the Lamp2-E1-3 EV + FUS groups were performed for each time point. ( E - F ) Fluorescence imaging of the brains and spinal cords obtained immediately after humane euthanasia, including (E) captured images and (F) quantification of EV signals. In (A) and (C) , one mouse in the PBS group ( n = 2) serving as the fluorescence background was reused in some image capture sessions to keep the layout consistent. All quantitative data are presented as the mean ± SD of all mice in each group. ns, non-significant. * p < 0.05, ** p < 0.01, *** p < 0.001

Article Snippet: Complementary (c)DNA of human Lamp2 variant B was purchased from OriGene (cat. no. RC200456, Rockville, MD, USA).

Techniques: In Vivo, Fluorescence, Imaging, Injection, Expressing